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Multi-Angle Light Scattering (MALS) Detector DAWN Series

Multi-Angle Light Scattering (MALS) Detector DAWN Series

High-precision measurement of absolute molecular weight in the range of hundreds to hundreds of millions Da for the characterization of polymers, proteins, and nanoparticles!

The DAWN series measures the molecular weight and size of macromolecules such as proteins with high precision, using multi-angle light scattering (MALS) without the need for calibration curves created from standard substances. It can be connected to HPLC and UPLC, enabling a wide range of applications. 【Features】 - DAWN (18-angle detector) offers high sensitivity and wide-range measurement. - A diverse lineup including DAWN8 (8-angle detector), miniDAWN (3-angle detector), microDAWN (UPLC-compatible model), and UltraDAWN (process-compatible model). - Automated cell cleaning with built-in ultrasonic cleaner COMET. - Equipped with a dynamic light scattering (DLS) module, allowing measurements from a radius of 0.5 nm (optional). - Real-time display of system status, with troubleshooting guidance functionality. By clicking [Send], you agree that the information provided on this site will be used and processed by Waters in accordance with Waters' Terms of Use and Privacy Notice. Terms of Use and Privacy Notice www.waters.com/legalandprivacy

  • Analytical Equipment and Devices
  • High Performance Liquid Chromatograph
  • Scattering Detector

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[Analysis Case] Simple Quantitative Analysis of Sugars

[Analysis Case] Simple Quantitative Analysis of Sugars

This is an example of a simple quantitative analysis of seven types of sugars converted to glucose equivalents using an evaporative light scattering detector (ELSD).

Sugars come in many types, and quantitative analysis to determine the sugar content in samples using standard substances for each type can become complicated, involving various tasks from pretreatment to measurement and analysis. Here, we introduce a case where an evaporative light scattering detector (ELSD) was used to simply quantify seven types of sugars by converting them to glucose equivalents. 【Analysis Samples】 Standard substances: 7 types of sugars (galactose, glucose, sucrose, trehalose, fructose, maltose, lactose) Samples: Commercial vegetable juices A and B, lactic acid bacteria drinks 【Analysis Method】 A mixture of the seven types of sugar standard substances was prepared, and HPLC-ELSD measurement was conducted. Using the conversion quantitative method based on glucose equivalents allows for a straightforward quantification of sugars in the samples. This method is effective when wanting to confirm the approximate concentration of sugars contained in various samples related to bio-manufacturing, such as food and beverages, cell and microbial culture media, and culture solutions.

  • Contract measurement
  • Scattering Detector

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Understand in 3 minutes! What is an evaporative light scattering detector? Free explanatory materials available.

Understand in 3 minutes! What is an evaporative light scattering detector? Free explanatory materials available.

What is an evaporative light scattering detector used in HPLC? A comprehensive explanation based on a comparison table with other detectors! Free basic knowledge materials available!

The evaporative light scattering detector (ELSD) detects the scattered light from particles of the analyte after evaporating and removing the mobile phase from the eluent coming from the column. Since the mobile phase must be volatile, mobile phases containing non-volatile salts such as phosphates cannot be used. Additionally, analytes that are highly volatile cannot be applied as they will evaporate along with the mobile phase. However, it is possible to detect compounds that do not have UV-Vis absorption or fluorescence. This document provides a clear summary of the fundamentals of liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training. [Contents (partial)] ■ Introduction ■ HPLC Equipment ■ Separation in HPLC ■ Detection *For more details, please download the PDF or feel free to contact us.

  • Analytical Equipment and Devices
  • Scattering Detector

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